[Lfm] CoVID-19
Isabel Palacios
i.palacios at qmul.ac.uk
Wed Mar 18 12:44:45 GMT 2020
a link to extracting RNA without kits
http://www.en-journal.org/journal/view.html?uid=513&vmd=Full
On 18/03/2020 12:43, Fernandes, Vilaiwan wrote:
> This too:
>
> From: Kathryn Harris <Kathryn.Harris at gosh.nhs.uk>
> Sent: 17 March 2020 09:30
> To: Vice Provost (Health) <viceprovosthealth at ucl.ac.uk>
> Subject: Request for help with coronavirus testing reagents
> Would to be possible to put the following request out across the UCL
> network?
> If anyone is using the following master mix: ‘SuperScript III One-Step
> RT-PCR System with Platinum Taq DNA Polymerase’ could they please
> contact me by email. This is to help with COVID-19 testing in
> Microbiology. Even small amounts would be potentially helpful.
> Kind Regards
> Kathryn
> Kathryn Harris PhD, FRCPath
> Principal Clinical Scientist
> Microbiology, Virology and Infection Prevention & Control
> Level 4 Camelia Botnar Laboratories
> Great Ormond Street Hospital NHS Foundation Trust
> London WC1N 3JH
> Tel: 020 7405 9200 x0437
>
> ------------------------------------------------------------------------
> *From:* Lfm <lfm-bounces at londonflymeeting.org> on behalf of Alex Gould
> <Alex.Gould at crick.ac.uk>
> *Sent:* Wednesday, March 18, 2020 12:34:56 PM
> *To:* Manolis Fanto <manolis.fanto at kcl.ac.uk>
> *Cc:* lfm at londonflymeeting.org <lfm at londonflymeeting.org>
> *Subject:* Re: [Lfm] CoVID-19
> Hi All,
>
> Below is an extract from an email I got requesting PCR volunteers from
> our labs on behalf of Public Health England. According to them, the
> sample swabs will already have been sterilised.
>
> Best wishes to you all at this tricky time,
>
> Alex
>
> To that we would be grateful if you could consult with your group and
> provide a list of any who would wish to volunteer in this way. As you
> may imagine there are all sorts of uncertainties that remain to be
> resolved, but the current position is broadly as follows:
>
> • The major current diagnostic bottleneck is PCR, therefore this call
> is for those competent in PCR methods – the NHS will be operating at
> clinical diagnostic standards and will of course provide training
> where needed
>
> • Many Crick laboratories could probably manage some secondment of lab
> members to diagnostic labs, so for instance a lab member could
> volunteer for 2 sessions per week (2 x 4hr) and still keep other Crick
> lab work going
>
> • There should not be major safety issues (virus should be sterilized
> prior to all procedures), but those that government has defined
> vulnerable groups should not volunteer (this would be in line with
> directions to those groups about home working)
>
> • There are a number of NHS laboratories which have indicated a need,
> so once we have a list of those who are interested, we will work to
> provide the most straightforward and protected (travel and other)
> arrangements – including compensating any expenses which you might incur
>
>
>
>> On 18 Mar 2020, at 12:27, Fanto, Manolis <manolis.fanto at kcl.ac.uk
>> <mailto:manolis.fanto at kcl.ac.uk>> wrote:
>>
>> I do have to say that I would not feel particularly safe with having
>> the average fly pusher manipulating swabs of potentially infective
>> material. I’d say this has to be done with all precautions by experts
>> in Cat2 or 3 facilities.
>> What other researchers could do is to receive isolated RNA and run
>> pcrs, not more than this.
>> I volunteer the Crick for that😉. As far as King’s and UCL go, we are
>> shutting down labs this week.
>> *From:*Lfm <lfm-bounces at londonflymeeting.org
>> <mailto:lfm-bounces at londonflymeeting.org>>*On Behalf Of*Isabel Palacios
>> *Sent:*18 March 2020 12:20
>> *To:*lfm at londonflymeeting.org <mailto:lfm at londonflymeeting.org>
>> *Subject:*Re: [Lfm] CoVID-19
>>
>> i wondered myself about this, but i am not sure... what then? we
>> should be isolating ourselves more now in any case? happy to discuss
>> more. i have a protocol for the rtpcr, as well as rna etxraction
>> protocols that dont need kits
>>
>> On 18/03/2020 12:16, Stefano Casalotti wrote:
>>
>> Dear All
>> this email is not about flies but CoVID-19. I am writing to this
>> forum because it is biggest forum I am in touch with.
>> I am wondering whether like me you are surprised about the
>> decision of the government to do minimal testing for the virus :
>> effectively they test only those that are ill and in hospital.
>> WHO is urging all countries to do as much testing as possible.
>> The scientific community would have a very large capacity to
>> carry out tests for the virus, and we could set up a system where
>> members of the public could send swabs to be tested. Of course
>> there are very major logistic steps to take but I think it is
>> case of 'where there is a will there is a way’
>> Probably this kind of thinking is happening in other online
>> groups (please point me to them if it is the case) but I was
>> wondering what anybody thinks about this and whether they would
>> be interested in forming a group prepared to take on such an
>> initiative.
>> Regards
>> Stefano
>>
>> Dr. Stefano Casalotti
>> Honorary Senior Lecturer
>> University of East London
>> School Health, Sport and Bioscience
>> Water Lane , London E15 4LZ
>> M: +44 (0)7763300261
>>
>>
>>
>> On 13 Mar 2020, at 11:55, Nic Tapon <Nic.Tapon at crick.ac.uk
>> <mailto:Nic.Tapon at crick.ac.uk>> wrote:
>> *This email is from an external source. Ensure you trust the
>> sender before opening any attachments or clicking on any links.*
>> ------------------------------------------------------------------------
>> Dear LFMers,
>> Sadly, we have taken the decision to*cancel the March and
>> April LFM*s, which will probably not come as a surprise to
>> all of you.
>> We will monitor the situation and will make a decision on the
>> May and June meetings so that we can update you as soon as
>> possible.
>> In the meantime, best wishes to all of you during these
>> trying times!
>> Kind regards,
>> Nic, Isabel and Lucy
>> /The Francis Crick Institute Limited is a registered charity
>> in England and Wales no. 1140062 and a company registered in
>> England and Wales no. 06885462, with its registered office at
>> 1 Midland Road London NW1 1AT/
>> _______________________________________________
>> Lfm mailing list
>> Lfm at londonflymeeting.org <mailto:Lfm at londonflymeeting.org>
>> http://lists.londonflymeeting.org/listinfo/lfm
>> <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885072286&sdata=%2FMZwODN8t2OnP1cMrx1Lr4oWKazzwSfmFlGSiFGdpJo%3D&reserved=0>
>>
>> <image001.png>
>>
>> <image002.jpg>
>> <https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Feu-api.mimecast.com%2Fs%2Fclick%2F8WiDjjiHHucTLIndY2LgTg-Z01eXn5Vg5DFcHPrCm-ir3s2gNztqTYcPE1XHgJmEVnVMVVSLtmVpJsG7RTepraYwWF0SDShWHA-J6VNYcoxXhMaj4aKop2Xk1OFTB7bA3rsYTKhHF33XpzrEemqkYoLoAypfb_z-kjgvYP7qGCjm2NOnkIjDxuB1TFG_1L85LQraFBlfKNA4IHY6CrPcKg&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885082279&sdata=a4rg00rHNQ7l0vMDxq5YmL%2FhrD2l7vV3oNVBbmvyMlo%3D&reserved=0>
>>
>>
>> <image003.jpg>
>> <https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Feu-api.mimecast.com%2Fs%2Fclick%2FuYdzQzQXhDdHR-C0Isqh0lx4Kz-dHOrLiYKYurlKooJvk8Il6lkBch41vEj9QBivGeq5fM-4t3Hos0Bb7zYQDm11lGiTulvwG2LK-O1FYNjPlHKzSJwTi-lKoNqfMMqxAfUgWFyqSdIYvbjB05l7ofiwc1wo4Y3PKp1NWB73G5UKEma0oUm50vjD9xDtAOusWeFAtuOXV6rqy3K_pKz1Kg&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885092274&sdata=wRJuoyudVYQmGWdz%2F7XP31c8fmGx%2FM6SMZWzuAv4dWs%3D&reserved=0>
>>
>>
>> <image004.jpg>
>> <https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Feu-api.mimecast.com%2Fs%2Fclick%2FF2A44qlyvx7D1oreXULOBVYbzWmseLyj-SEsW-0xIuL0lvm8hz32DgnE3toX353PjKO-lJAszRb3gFU8_Zy9InVPCRBYg4JXq_Wd9owjxjcmdq_H48sUzXXVFqIe_PqbLaSpsKcVvdejoM0VhDpJ3Lz-NyKXzT_obsc2HO_EEmmvTw7Ak3FiYAKWJ1vjGgIu6JjKB4ElMvCYMrrwYneayw&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885092274&sdata=AJfhAnZ0rI3LgQfT8TAQ0KpvsW0XZ6GFsqsvDC9hP8o%3D&reserved=0>
>>
>>
>> <image005.jpg>
>> <https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Feu-api.mimecast.com%2Fs%2Fclick%2FqOmVl5rbG-c8AKFGL4MX7UIjM3wg8VH5THWNyKUTqILyxnncpVv18qPPuqXO732-jJfS49dT4qIKPbYbdiYdm25su8nL514UNAItWccVRcxGO6t7MBDyT0L54blCQ-6iaYjhL4drJKD0ha4mT1bJXnjleuwQmTZqMLTB4PiU6TZZe7ysxd-sBQiYkpu-Bnzycsh7ZMT24A-1kWBNWnZtOg&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885102270&sdata=k%2BGt4UHML7o%2Bw%2F7SbrUtBL0asw%2F23mUgCyiAw5HmlUk%3D&reserved=0>
>>
>>
>> The information transmitted in this e-mail and its contents
>> isintended only for the person or entity to which it is addressed
>> and may contain confidential and/or privileged material. If you
>> are not the intended addressee you are prohibited from storing,
>> copying or using the information in any way. This email has been
>> checked for viruses and malware but no liability is accepted by
>> UEL for any damage caused by any virus or malware that may be
>> transmitted by this email.
>>
>>
>>
>> _______________________________________________
>>
>> Lfm mailing list
>>
>> Lfm at londonflymeeting.org <mailto:Lfm at londonflymeeting.org>
>>
>> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C394ae9c4ffd04ddebda108d7cb3644ee%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201306282105825&sdata=sqTZtD%2BAN8Do6GJ%2FAG4qhz60cXHp32zaXAWWkfLRpb8%3D&reserved=0 <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885102270&sdata=znv8XNK20yyk8RoDeUYyDWu5NQxDLhqs4Efp0PomDcs%3D&reserved=0>
>>
>> --
>> Isabel M. Palacios
>> Lecturer in Cell Biology
>> School of Biological & Chemical Sciences
>> Queen Mary University of London
>> Scientific visitor at the University of Cambridge
>> Founder of DrosAfricahttp://drosafrica.org <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fdrosafrica.org%2F&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885112264&sdata=eIxxbTv6FgPr4qCGyzVRjHMrwuXqO5n1KypydEicG4w%3D&reserved=0>
>> BSCB Ambassadorhttp://bscb.org <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fbscb.org%2F&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885112264&sdata=Hm5NloXlbaFnh6qBLuLsu5kWWLmb5v%2FiA1g2yFrthdg%3D&reserved=0>
>> _______________________________________________
>> Lfm mailing list
>> Lfm at londonflymeeting.org <mailto:Lfm at londonflymeeting.org>
>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C79bab4df78aa42dedfef08d7cb37b82e%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C0%7C637201312518126165&sdata=JN87krOMyk46x3FTVXoTwcdSzm7HdpdNwoErNXphTIc%3D&reserved=0
>> <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885122261&sdata=7ao1bordCx3ulyDU40iEhg%2BBVz5ucVXozHpEjI4x44s%3D&reserved=0>
>
> ************************
>
> Alex P. Gould PhD FMedSci,
> Senior Group Leader,
> Wellcome Trust Investigator,
> The Francis Crick Institute,
> 1 Midland Road,
> London NW1 1AT
> UK
>
> (courier deliveries: The Francis Crick Institute, 1 Brill Place,
> London NW1 1BF. UK)
>
> Phone: +44 (0)20 37961452
>
> Lab Administrator:
> Anika Tailor
> anika.tailor at crick.ac.uk <mailto:anika.tailor at crick.ac.uk>
> Phone: +442037965330
>
> http://www.agouldlab.com
> <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.agouldlab.com%2F&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885122261&sdata=Znb9YfoaanaFU67amLGw0DBVZbU%2Fk2zbkVC2br9Q0zM%3D&reserved=0>
> http://www.crick.ac.uk/alex-gould
> <https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.crick.ac.uk%2Falex-gould&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885132251&sdata=9rkkHtp36IXkc%2BQEOmtVCI9etPUT7q%2FV25FyhH5UKJo%3D&reserved=0>
>
>
>
> The Francis Crick Institute Limited is a registered charity in England
> and Wales no. 1140062 and a company registered in England and Wales
> no. 06885462, with its registered office at 1 Midland Road London NW1 1AT
>
>
> _______________________________________________
> Lfm mailing list
> Lfm at londonflymeeting.org
> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.londonflymeeting.org%2Flistinfo%2Flfm&data=02%7C01%7C%7C7f943c6c2aba4f142d6108d7cb39e747%7C569df091b01340e386eebd9cb9e25814%7C0%7C0%7C637201321885152243&sdata=ULcavbomjITjtG1OqDaMcrvIRsR45RxUEBz0fyIdQ44%3D&reserved=0
--
Isabel M. Palacios
Lecturer in Cell Biology
School of Biological & Chemical Sciences
Queen Mary University of London
Scientific visitor at the University of Cambridge
Founder of DrosAfrica http://drosafrica.org
BSCB Ambassador http://bscb.org
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.londonflymeeting.org/archives/lfm/attachments/20200318/e00bc2e9/attachment-0001.htm>
More information about the Lfm
mailing list