[Lfm] Recycling fly vials

Wed Dec 8 08:22:19 UTC 2021

Hi All,

@Annick: Stefano has asked me to reply regarding the flushing of vials. I connected a tube with a relatively small lumen diameter to a regular lab tap to create high pressure. It took seconds to disturb the food and flush the vial. Some stubborn pupae remained but after soaking in a lab decontaminating solution for at least a few hours (previously used Distel and ChemGene), when rinsing the decon away, the remaining pupae was always removed. For heavily pupated bottles, a brief wash with a brush like this<https://www.google.com/search?q=small+bottle+brush&rlz=1C1GCEV_enGB956GB956&source=lnms&tbm=isch&sa=X&sqi=2&ved=2ahUKEwixjPT64dP0AhULoXIEHWetAlcQ_AUoAnoECAEQBA&biw=1920&bih=937&dpr=1> when doing the initial vial flush helped. I have also used a small pressure water sprayer<https://www.google.com/search?q=water+pressure+sprayer&tbm=isch&ved=2ahUKEwjzqsaE4tP0AhVCwYUKHduMA20Q2-cCegQIABAA&oq=water+pressure+sprayer&gs_lcp=CgNpbWcQAzIFCAAQgAQyBggAEAgQHjIGCAAQCBAeMgYIABAIEB4yBggAEAgQHjIECAAQGDIECAAQGDIECAAQGDIECAAQGDoECAAQQzoICAAQgAQQsQM6CAgAELEDEIMBOgcIABCxAxBDOgYIABAFEB5QmwVY6Sdg6ShoAHAAeACAAUKIAcAJkgECMjOYAQCgAQGqAQtnd3Mtd2l6LWltZ8ABAQ&sclient=img&ei=NGqwYfPAJsKClwTbmY7oBg&bih=937&biw=1920&rlz=1C1GCEV_enGB956GB956> but honestly the pipe on a regular lab tap works just as well.

Cheers,
Dan

Dr Daniel Ranson
Postdoctoral Research Associate- Bateman Lab
Maurice Wohl Clinical Neuroscience Institute, 3.13.17
Department of Basic and Clinical Neuroscience
King's College London
5 Cutcombe Road
London, SE5 9RT, UK

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From: Lfm <lfm-bounces at londonflymeeting.org> On Behalf Of Annick Sawala
Sent: 06 December 2021 11:04
Cc: lfm at londonflymeeting.org
Subject: Re: [Lfm] Recycling fly vials

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Hi all,

@Stefano: just wondering, what do you use to flush out the food? Just a regular lab tap with tubing, or something with more force?

in response to Rita's question regarding re-usable labels:

we found electrical tape works very well - we ordered from here: https://www.gaffatape.com/<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.gaffatape.com%2F&data=04%7C01%7CDaniel.ranson%40kcl.ac.uk%7C7806a71684bf4abe3c6808d9b8a81470%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C637743855358210035%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=syT9eW%2F3f1nZCtWsnW%2B2m%2Fd66UEbaNEk4WYa0e2Yw9o%3D&reserved=0>
I'm pasting our order details from a while ago:
2 x Electrical PVC Insulation Tape - Single Rolls (LEMPVC25G) at £1.04 = £2.07
2 x Electrical PVC Insulation Tape - Single Rolls (LEMPVC25R) at £1.04 = £2.07
2 x Electrical PVC Insulation Tape - Single Rolls (LEMPVC25Y) at £1.04 = £2.07
2 x Electrical PVC Insulation Tape - Single Rolls (LEMPVC19B) at £0.78 = £1.57
2 x Electrical PVC Insulation Tape - Single Rolls (LEMPVC19PK) at £0.78 = £1.57

We then print stock labels onto Avery labels L7656 (can be imported from an excel file).

After sticking the label onto a piece of electrical tape, we cover it with Scotch crystal clear tape.

They take a little time to make but last several years.

All the best,
Annick

On Fri, Dec 3, 2021 at 10:03 AM Damien Goutte-Gattat <dpg44 at cam.ac.uk<mailto:dpg44 at cam.ac.uk>> wrote:
Hi,

> On 3 Dec 2021, at 08:41, James Hodge <James.Hodge at bristol.ac.uk<mailto:James.Hodge at bristol.ac.uk>> wrote:
> I am looking into decreasing the consumption of one use plasticware for flies in the lab, has or is anybody recycling their plastic or using glass, etc? If so, please can you email me how you do it/advice?

If you have not seen it already, someone from an Argentina fly lab recently shared on Twitter her protocol for washing and reusing fly vials. [1]

Best,

- Damien

[1] https://twitter.com/MuraroNara/status/1458912682678145024<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Ftwitter.com%2FMuraroNara%2Fstatus%2F1458912682678145024&data=04%7C01%7CDaniel.ranson%40kcl.ac.uk%7C7806a71684bf4abe3c6808d9b8a81470%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C637743855358210035%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=SHqIRRe8L77OrC16FEWraP5klvn83vkG33ajI5DQaFE%3D&reserved=0>

--
Damien Goutte-Gattat, PhD   Department of Physiology,
                             Development and Neuroscience
dpg44 at cam.ac.uk<mailto:dpg44 at cam.ac.uk>             Downing Street
+44 7954 894097             Cambridge CB2 3DY

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--
Annick Sawala, PhD
Senior Scientist
Vivan Therapeutics (formerly My Personal Therapeutics)
vivantx.com<https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fvivantx.com%2F&data=04%7C01%7CDaniel.ranson%40kcl.ac.uk%7C7806a71684bf4abe3c6808d9b8a81470%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C637743855358210035%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=%2FGvF3s8%2BFI7eySKsztI2GbSzrXII00ddff78r8%2BPXVI%3D&reserved=0>
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