[Lfm] phospho-Akt antibody for Drosophila

[Dionne, Marc S]

Hi Paulo (and all)—

We’ve previously used D9E (the recommended substitution) on whole-fly westerns and gotten basically identical results to what we got with 4054. If D9E is now not working, I don’t know what we would use instead…

Yours
Marc

> On 7 Jan 2025, at 15:47, Paulo Baptista-Ribeiro <p.baptista-ribeiro at qmul.ac.uk> wrote:
> 
> This email from p.baptista-ribeiro at qmul.ac.uk originates from outside Imperial. Do not click on links and attachments unless you recognise the sender. If you trust the sender, add them to your safe senders list to disable email stamping for this address.  Dear LFMers,
> 
> We are looking for a phospho-Akt antibody for Drosophila. We currently have a very small aliquot of a p-Akt antibody from CST (#4054;https://www.cellsignal.com/products/primary-antibodies/phospho-drosophila-akt-ser505-antibody/4054), which has been discontinued.
> 
> The suggested replacement for the discontinued antibody is CST #4060 (https://www.cellsignal.com/products/primary-antibodies/phospho-akt-ser473-d9e-xp-rabbit-mab/4060), which is supposed to cross-react with the Drosophila protein in Western blots but we didn't see this in our hands.
> 
> If anyone has or knows of an alternative p-Akt that works with Drosophila (ideally in both IF and WB), please let us know.
> 
> Thank you very much for your help.
> 
> All the best,
> Paulo
> 
> ____________________________________________________________________________
> Paulo S. Ribeiro
> Reader in Cell and Developmental Biology | Protein Dynamics and Cell Signalling Laboratory | Centre for Tumour Biology
> Barts Cancer Institute - Queen Mary University of London and part of the Cancer Research UK City of London Centre
> T: +44 (0)20 7882 3829 | E: p.baptista-ribeiro at qmul.ac.uk
> https://www.bci.qmul.ac.uk/
> <Outlook-d1g01edc.png>
> 
> *******************
> This email originates from outside Imperial. Do not click on links and attachments unless you recognise the sender. 
> If you trust the sender, add them to your safe senders list https://spam.ic.ac.uk/SpamConsole/Senders.aspx to disable email stamping for this address.
> *******************
> _______________________________________________
> Lfm mailing list
> Lfm at londonflymeeting.org
> http://lists.londonflymeeting.org/listinfo/lfm




-- 
Marc S Dionne, PhD
Principal Investigator, Centre for Bacterial Resistance Biology
Professor of Innate Immunity
Department of Life Sciences
Imperial College London
South Kensington Campus
G.23 Flowers Building
London SW7 2AZ
United Kingdom
+44 20 7594 5776
http://www.imperial.ac.uk/people/m.dionne