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Dear LFMers,</div>
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We are looking for a phospho-Akt antibody for Drosophila. We currently have a very small aliquot of a p-Akt antibody from CST (#4054;
<a href="https://www.cellsignal.com/products/primary-antibodies/phospho-drosophila-akt-ser505-antibody/4054" id="LPlnk130420" class="OWAAutoLink">
https://www.cellsignal.com/products/primary-antibodies/phospho-drosophila-akt-ser505-antibody/4054</a>), which has been discontinued.</div>
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The suggested replacement for the discontinued antibody is CST #4060 (<a href="https://www.cellsignal.com/products/primary-antibodies/phospho-akt-ser473-d9e-xp-rabbit-mab/4060" id="LPlnk726284" class="OWAAutoLink">https://www.cellsignal.com/products/primary-antibodies/phospho-akt-ser473-d9e-xp-rabbit-mab/4060</a>),
which is supposed to cross-react with the Drosophila protein in Western blots but we didn't see this in our hands.</div>
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If anyone has or knows of an alternative p-Akt that works with Drosophila (ideally in both IF and WB), please let us know.</div>
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Thank you very much for your help.</div>
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All the best,</div>
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Paulo</div>
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<span style="font-family: Arial, sans-serif; font-size: 10pt; color: black;">Paulo S. Ribeiro</span></p>
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<span style="font-family: Arial, sans-serif; font-size: 10pt; color: black;">Reader in Cell and Developmental Biology | Protein Dynamics and Cell Signalling Laboratory | Centre for Tumour Biology</span></p>
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<span style="font-family: Arial, sans-serif; font-size: 10pt; color: black;">Barts Cancer Institute - Queen Mary University of London and part of the Cancer Research UK City of London Centre</span></p>
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<span style="font-family: Arial, sans-serif; font-size: 10pt; color: black;">T: +44 (0)20 7882 3829 | E:
</span><span style="font-family: Arial, sans-serif; font-size: 10pt; color: rgb(5, 99, 193);"><a href="mailto:p.baptista-ribeiro@qmul.ac.uk" target="_blank" id="OWAb683019f-a778-e053-be97-2bcb8c151f8f" class="OWAAutoLink" rel="noopener noreferrer" style="color: rgb(5, 99, 193); margin: 0px;">p.baptista-ribeiro@qmul.ac.uk</a></span><span style="font-family: Arial, sans-serif; font-size: 10pt; color: black;"><br>
</span><span style="font-family: Arial, sans-serif; font-size: 10pt; color: rgb(5, 99, 193);"><a href="https://www.bci.qmul.ac.uk/" target="_blank" id="OWA45edb3c4-9218-88f3-f9f8-0528317d7923" class="OWAAutoLink" rel="noopener noreferrer" style="color: rgb(5, 99, 193); margin: 0px;">https://www.bci.qmul.ac.uk/</a></span></p>
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