[Lfm] Shields and Sang M3 medium

[Nic Tapon]

Hi LFMers,
See the message below from London Fly community member Rippei Hayashi about the disappearance of Shields and Sang M3 medium.
If any you you have a solution, please get in touch with him!
All the best,
Nic
From: "Hayashi,Rippei" <rippei.hayashi at imba.oeaw.ac.at<mailto:rippei.hayashi at imba.oeaw.ac.at>>
Date: Tuesday, 5 December 2017 at 20:31
To: Nic Tapon <nic.tapon at crick.ac.uk<mailto:nic.tapon at crick.ac.uk>>
Subject: Shields and Sang M3 medium

Dear Nic

I am writing you because I would like to circulate an inquiry email in the fly community in London.
Our lab heavily uses a cell line derived from follicle cells called Ovarian Somatic Cells, OSCs in short.
They are delicate and we have only been able to culture them with the Shields and Sang M3 medium.
But, recently, Sigma and other producers stopped producing this medium and they don’t know when they will start making it again.

I wonder if anyone experienced the same and found an alternative or could recommend similar medium to try.
We tried the Schneider’s medium, but it did not work.

I would be grateful if you could circulate my email to the London fly community.

Many thanks
Rippei

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Rippei Hayashi, PhD
Postdoc
Julius Brennecke's lab
IMBA - Institute of Molecular Biotechnology
Dr. Bohr-Gasse 3
1030 Vienna, Austria
Tel: +43 1 79044 4507
email: rippei.hayashi at imba.oeaw.ac.at<mailto:rippei.hayashi at imba.oeaw.ac.at>
-----


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