[Lfm] Alternative to fly pooter

[Isabel Palacios]

Don’t know if it will help but there are some plastic pipettes for liquid that may work. With a little extended area at the top. Don’t know how they are called

Sent from my iPhone

> On 6 Nov 2020, at 14:41, kofan <kofanchen at gmail.com> wrote:
> 
> 
> Hi Joe,
> Just came up with this setting (see picture) by replacing the mouth with a 50ml syringe, it works for suction but for blowout it seems to need so twigging but this may be a starting point?
> 
> Hope this helps
> Kofan
>  
>>> On Fri, 6 Nov 2020, 14:18 Bateman, Joseph Matthew, <joseph_matthew.bateman at kcl.ac.uk> wrote:
>>> Dear Fly folks,
>>> 
>>>  
>>> 
>>> Does anyone know of an alternative to a fly pooter (aka aspirator), that doesn’t involve mouth pipetting, which works for moving around individual flies prior to behavioural experiments? We have always used a pooter but have recently been obliged to stop as it involves mouth pipetting. We can cool the flies to temporarily anaesthetise them (or obviously use CO2) but this may well affect their behaviour, especially for more immediate assays.
>>> 
>>>  
>>> 
>>> If you want to email me direct (Joseph_matthew.bateman at kcl.ac.uk) that might be best to prevent jamming up people’s inboxes. I can always send out a list of responses.
>>> 
>>>  
>>> 
>>> Any suggestions would be appreciated! 
>>> 
>>>  
>>> 
>>> Thanks,
>>> 
>>>  
>>> 
>>> Joe
>>> 
>>>  
>>> 
>>> Joseph Bateman
>>> 
>>> Reader in Molecular Neuroscience
>>> 
>>> Maurice Wohl Clinical Neuroscience Institute
>>> 
>>> Department of Basic and Clinical Neuroscience
>>> 
>>> King’s College London
>>> 
>>> 125 Coldharbour lane
>>> 
>>> London
>>> 
>>> SE5 9NU
>>> 
>>> UK
>>> 
>>> Joseph_matthew.bateman at kcl.ac.uk
>>> 
>>> https://www.batemanlab.org
>>> 
>>> https://twitter.com/BatemanLab
>>> 
>>> 44-(0)207 848 8144
>>> 
>>>  
>>> 
>>> 
>>> 
>>>  
>>> 
>>> _______________________________________________
>>> Lfm mailing list
>>> Lfm at londonflymeeting.org
>>> http://lists.londonflymeeting.org/listinfo/lfm
> 
> <DSC_0039.JPG>
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