[Lfm] Alternative to fly pooter

[kofan]

 Hi Joe,
Just came up with this setting (see picture) by replacing the mouth with a
50ml syringe, it works for suction but for blowout it seems to need so
twigging but this may be a starting point?

Hope this helps
Kofan


> On Fri, 6 Nov 2020, 14:18 Bateman, Joseph Matthew, <
> joseph_matthew.bateman at kcl.ac.uk> wrote:
>
>> Dear Fly folks,
>>
>>
>>
>> Does anyone know of an alternative to a fly pooter (aka aspirator), that
>> doesn’t involve mouth pipetting, which works for moving around individual
>> flies prior to behavioural experiments? We have always used a pooter but
>> have recently been obliged to stop as it involves mouth pipetting. We can
>> cool the flies to temporarily anaesthetise them (or obviously use CO2) but
>> this may well affect their behaviour, especially for more immediate assays.
>>
>>
>>
>> If you want to email me direct (Joseph_matthew.bateman at kcl.ac.uk) that
>> might be best to prevent jamming up people’s inboxes. I can always send out
>> a list of responses.
>>
>>
>>
>> Any suggestions would be appreciated!
>>
>>
>>
>> Thanks,
>>
>>
>>
>> Joe
>>
>>
>>
>> Joseph Bateman
>>
>> Reader in Molecular Neuroscience
>>
>> Maurice Wohl Clinical Neuroscience Institute
>>
>> Department of Basic and Clinical Neuroscience
>>
>> King’s College London
>>
>> 125 Coldharbour lane
>>
>> London
>>
>> SE5 9NU
>>
>> UK
>>
>> Joseph_matthew.bateman at kcl.ac.uk
>>
>> https://www.batemanlab.org
>>
>> https://twitter.com/BatemanLab
>>
>> 44-(0)207 848 8144
>>
>>
>>
>>
>> _______________________________________________
>> Lfm mailing list
>> Lfm at londonflymeeting.org
>> http://lists.londonflymeeting.org/listinfo/lfm
>>
>
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